3. Date of birth: 28/10/1988 4. Place of birth: Hanoi
5. Admission decision number: No. 134/QĐ-CNSH dated on 27/07/2016 by Director of Institute of Microbiology and Biotechnology
6. Changes in academic process: Extend study period (decision number: No. 147/QĐ-CNSH date on 02/07/2019 and No. 17/QĐ-CNSH date on 16/01/2020 by Director of Institute of Microbiology and Biotechnology)
7. Official thesis title: “Study on sulfate reducing bacteria for the application in treatment of acidic mine drainage containing high concentrations of heavy metals and arsenic”
8. Major: Biotechnology 9. Code: 62 42 02 01
10. Supervisors: 1) Assoc. Prof. Dr. Nguyen Lan Huong
2) Dr. Dinh Thuy Hang
11. Summary of the new findings of the thesis
This is the first detailed study in Vietnam about acid- and metal-tolerant sulfate-reducing bacteria (SRB). The most important scientific contributions of the study include:
Acid-tolerant sulfate reducing strain Desulfovibrio oxamicus S4 was successfully obtained. This strain has valuable biological properties suitable for application in AMD treatment such as:
Grow and reduce sulfate in acidic environments (pH 2 and above)
Resist high concentrations of heavy metals
Simultaneously reduce sulfate and arsenate to remove arsenic species in the form of As2S3 precipitate.
Initiate the sulfate reduction in AMD environment, facilitating growth conditions for common SRB species.
The effect of Microbial product SRA containing living cells of the strain Desulfovibrio oxamicus S4 encapsulated in alginate has demonstrated in early start-up of sulfate reduction and effective treatment of AMD in a Lab-scaled system (using synthetic AMD) and a pilot system (using AMD from Thien Ke tin processing factory).
12. Paratical applicability, if any: The strain Desulfovibrio oxamicus S4 obtained from this study would be used as an SRB source (Microbial product SRA) for the treatment of AMD suitable to the conditions in Vietnam.
13. Further research directions, if any: Study and develop technological process according to the module for treatment of heavy metal-contaminated wastewater from mining and mineral processing activities including metal mines, metal processing factories, and metal recycling craft villages... using the strain Desulfovibrio oxamicus S4 (Microbial product SRA) as a SRB source.
14. Thesis-related publications:
[1] Nguyen T.H., Nguyen L.H., Nguyen H.M., Nguyen K.N.T., Dinh T.H. (2020), “Sulfate Reduction for Bioremediation of AMD Facilitated by an Indigenous Acid- and Metal-tolerant Sulfate-Reducer”, J. Microbiol. Biotechnol. 30(7), pp. 1005-1012.
[2] Dinh T.H., Nguyen T.H. (2018), Strain Desulfovibrio oxamicus S4 is biologically pure for application in acid mine drainage treatment, Patent (No. 20127). Decision No. 76715/QĐ-SHTT dated October 30, 2018.
[3] Nguyen T.H., Nguyen L.H., Dinh T.H. (2018), “Finding arsenate-reducing bacteria for treatment of arsenic contaminated wastewater”, National Conference on Biotechnology, pp. 1078-1084.
[4] Nguyen T.H., Dinh T.H. (2016), “Elimination of iron from acidic mine drainage (AMD) in the co-treatment with poultry wastewater through a sulfate reducing bioreactor. Vietnam Journal of Biotechnology 14(2), pp. 369-375.
[5] Nguyen T.H., Dinh T.H. (2016), “Treatment of acidic wastewater from Thien Ke tin processing factory by sulfate reducing bioreactor: Pilot scale study”, Vietnam Journal of Biotechnology 14(4), pp. 777-784.
