3. Date of birth: 21/3/1985 4. Place of birth: Nam dinh
5. Admission decision number: 4860/QĐ-ĐHKHTN of Director of VNU University of Science
6. Changes in academic process: extension of the study in 2017, 2018
7. Official thesis title: Studying of methods for simultaneous determination of anthocyanins and anthocyanidins in vegetables, tubers, fruits.
8. Major: analytical chemistry 9. Code: 9440112.03
10. Supervisors: Assoc. Prof, PhD Le Thi Hong Hao and Assoc. Prof, PhD Nguyen Xuan Trung
11. Summary of the new findings of the thesis
- The thesis used the second experimental design method to find the optimal conditions for the liquid chromatographic separation to simultaneous determination of six anthocyanins and six anthocyanidins. These results were more optimal than those published using univariate methods. Simultaneous determination of both anthocyanins and anthocyanidins allows identification and quantification of both forms in a running time which helps to evaluate the hydrolysis recovery. Therefore, quantitative results were accuracy.
- The separation and extraction of anthocyanidin in vegetables, tubers and fruits were optimized using the second experimental design method. The results of the study show that the optimal conditions of the sample preparation when evaluating the simultaneous and reciprocal influence of the factors.
12. Practical applicability, if any:
- The optimal parameters of the HPLC-DAD for simultaneous determination of anthocyanins and anthocyanidins will be applied in laboratories which evalute recovery of extraction and hydrolysis for each specific sample matrix. It especially is useful when the standards source anthocyanins are not available.
- 31 kinds of vegetable, tuber and fruits have identified and quantified anthocyanidin. The study preliminary evaluated antioxidant activity of some food samples. From those results, manufactures can select raw materials with high anthocyanin content and biological activity.
- MS/MS data of anthocyanins and anthocyanidins contributes to providing more data for the spectral library to be applied for further studies.
13. Further research directions, if any
- Developing liquid chromatography tandem mass spectrometry (LC-MS) method to identify anthocyanin types in the vegetables, roots and fruits matrices.
- Studying the extraction and separation process to collect anthocyanin pigments from plants
- Assess the relationship between anthocyanidin ingredients, anthocyanidin content and antioxidant activity.
14. Thesis-related publications:
[1]. Lê Việt Ngân, Vũ Thị Trang, Lê Thị Hồng Hảo, Nguyễn Xuân Trung, Lê Đình Chi (2016), Xây dựng quy trình định lượng đồng thời 3 anthocyanidin trong một số loại rau, củ, quả bằng kỹ thuật HPLC, Tạp chí Dược học, số 484, trang 26-30.
[2]. Vũ Thị Trang, Lê Thị Hồng Hảo, Nguyễn Xuân Trung, Tăng Thị Phương (2016), Xác định đồng thời 5 anthocyanidin trong một số loại rau, củ, quả bằng phương pháp sắc ký lỏng khối phổ UPLC-MS/MS, Tạp chí Khoa học Đại học quốc gia hà nội, tập 32, số 4, trang 305-309.
[3]. Vũ Thị Trang, Chu Thị Thanh, Lê Thị Hồng Hảo, Nguyễn Xuân Trung (2018), Tối ưu hóa điều kiện thủy phân anthocyanin trong đỗ đen Việt nam sử dụng phương pháp mặt mục tiêu, Tạp chí phân tích Hóa lý và sinh học, T23, số 2, trang 42-48.
[4]. Vũ Thị Trang, Lê Thị Hồng Hảo, Nguyễn Đức Hảo, Nguyễn Hoài Thu, Lê Hoàng Đức, Nguyễn Xuân Trung (2018), Xác định tính chống oxy hóa của một số anthocyanin và anthocyanidin bằng phương pháp đo quang sử dụng phản ứng với 2,2’-diphenyl-1-picrylhydrazyl (DPPH), Tạp chí phân tích hóa, lý và sinh học, tập 23, số 5, trang 33-38.
[5]. Vu Thi Trang, Le Hoang Duc, Nguyen Hoai Thu, Le Thi Hong Hao, Nguyen Xuan Trung, Multiseparation of anthocyanins and anthocyanidins by high performance liquid chromatography combined with response surface methodology, Health Risk Analysis, 2019, no. 3, pp. 118–127. DOI: 10.21668/health.risk/2019.3.14.eng
